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permeabilization working solution  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc permeabilization working solution
    Permeabilization Working Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilization+working+solution/Cell+Permeabilization+Buffer/pmc12754057-474-22-92
    Average 95 stars, based on 205 article reviews
    permeabilization working solution - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: The Energy Sensor AMPKα1 Is Critical in Rapamycin-Inhibition of mTORC1-S6K-Induced T-cell Memory
    Article Snippet: Intracellular staining was done as per the manufacturer’s (BD Bioscience) protocol for flow cytometry. .. Briefly, after cell-surface staining, T-cells were fixed and permeabilized in 1 mL of fixation and permeabilization working solution for 20 min on ice, followed by a wash in 1X permeabilization solution and incubation with primary antibodies against T-bet, FOXO1, and pS6 (S 235/236 ) (Cell Signaling, Danvers, MA, USA) in 100 μL of 1X permeabilization buffer. .. Cells were washed once with 1 mL of 1X permeabilization solution, followed by incubation with PE-goat anti-rabbit IgG secondary antibody (Biolegend) in 100 μL of 1X permeabilization buffer.

    Article Title: The Energy Sensor AMPKα1 Is Critical in Rapamycin-Inhibition of mTORC1-S6K-Induced T-cell Memory.
    Article Snippet: Intracellular staining was done as per the manufacturer’s (BD Bioscience) protocol for flow cytometry. .. Briefly, after cell-surface staining, T-cells were fixed and permeabilized in 1 mL of fixation and permeabilization working solution for 20 min on ice, followed by a wash in 1X permeabilization solution and incubation with primary antibodies against T-bet, FOXO1, and pS6 (S235/236) (Cell Signaling, Danvers, MA, USA) in 100 μL of 1X permeabilization buffer. .. Cells were washed once with 1 mL of 1X permeabilization solution, followed by incubation with PE-goat anti-rabbit IgG secondary antibody (Biolegend) in 100 μL of 1X permeabilization buffer.

    Article Title: Prosurvival IL-7-Stimulated Weak Strength of mTORC1-S6K Controls T Cell Memory via Transcriptional FOXO1-TCF1-Id3 and Metabolic AMPKα1-ULK1-ATG7 Pathways.
    Article Snippet: For intracellular staining, a Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA) was used according to the manufacturer’s instructions. .. Briefly, after cell surface staining, T cells were fixed and permeabilized in 1 ml of fixation and permeabilization working solution for 1 h on ice, followed by a wash in 1 permeabilization solution and incubation with primary Abs against TCF1 and FOXO1 (Cell Signaling Technology, Danvers, MA) in 100 ml of 1 permeabilization solution. .. Cells were washed once with 1 ml of 1 permeabilization solution, followed by an incubation with PE goat anti-rabbit IgG secondary Ab (BioLegend) in 100 ml of 1 permeabilization solution.

    Incubation:

    Article Title: The Energy Sensor AMPKα1 Is Critical in Rapamycin-Inhibition of mTORC1-S6K-Induced T-cell Memory
    Article Snippet: Intracellular staining was done as per the manufacturer’s (BD Bioscience) protocol for flow cytometry. .. Briefly, after cell-surface staining, T-cells were fixed and permeabilized in 1 mL of fixation and permeabilization working solution for 20 min on ice, followed by a wash in 1X permeabilization solution and incubation with primary antibodies against T-bet, FOXO1, and pS6 (S 235/236 ) (Cell Signaling, Danvers, MA, USA) in 100 μL of 1X permeabilization buffer. .. Cells were washed once with 1 mL of 1X permeabilization solution, followed by incubation with PE-goat anti-rabbit IgG secondary antibody (Biolegend) in 100 μL of 1X permeabilization buffer.

    Article Title: The Energy Sensor AMPKα1 Is Critical in Rapamycin-Inhibition of mTORC1-S6K-Induced T-cell Memory.
    Article Snippet: Intracellular staining was done as per the manufacturer’s (BD Bioscience) protocol for flow cytometry. .. Briefly, after cell-surface staining, T-cells were fixed and permeabilized in 1 mL of fixation and permeabilization working solution for 20 min on ice, followed by a wash in 1X permeabilization solution and incubation with primary antibodies against T-bet, FOXO1, and pS6 (S235/236) (Cell Signaling, Danvers, MA, USA) in 100 μL of 1X permeabilization buffer. .. Cells were washed once with 1 mL of 1X permeabilization solution, followed by incubation with PE-goat anti-rabbit IgG secondary antibody (Biolegend) in 100 μL of 1X permeabilization buffer.

    Article Title: Avian GCGR-mediated continuous fat utilization offers perspectives for obesity treatment
    Article Snippet: .. Slides were washed in PBS (pH = 7.4) on a decolorizing shaker for 3 times 5 min. After addition of 50–100 μl permeabilization working solution, the following steps were: incubation at room temperature for 20 min, 3 washes with PBS, each for 5 min. A few drops of blocking buffer (10% donkey serum blocking buffer for incubation with goat-derived primary antibodies, or 3% BSA blocking buffer for incubation with primary antibodies from other species) were added, and incubation was carried out at room temperature for 30 min. Pre-prepared primary antibody anti-v5 (1:200, CST, USA) was added, and the slides were incubated flat in a humidified chamber at 4 °C overnight. ..

    Article Title: Prosurvival IL-7-Stimulated Weak Strength of mTORC1-S6K Controls T Cell Memory via Transcriptional FOXO1-TCF1-Id3 and Metabolic AMPKα1-ULK1-ATG7 Pathways.
    Article Snippet: For intracellular staining, a Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA) was used according to the manufacturer’s instructions. .. Briefly, after cell surface staining, T cells were fixed and permeabilized in 1 ml of fixation and permeabilization working solution for 1 h on ice, followed by a wash in 1 permeabilization solution and incubation with primary Abs against TCF1 and FOXO1 (Cell Signaling Technology, Danvers, MA) in 100 ml of 1 permeabilization solution. .. Cells were washed once with 1 ml of 1 permeabilization solution, followed by an incubation with PE goat anti-rabbit IgG secondary Ab (BioLegend) in 100 ml of 1 permeabilization solution.

    Blocking Assay:

    Article Title: Avian GCGR-mediated continuous fat utilization offers perspectives for obesity treatment
    Article Snippet: .. Slides were washed in PBS (pH = 7.4) on a decolorizing shaker for 3 times 5 min. After addition of 50–100 μl permeabilization working solution, the following steps were: incubation at room temperature for 20 min, 3 washes with PBS, each for 5 min. A few drops of blocking buffer (10% donkey serum blocking buffer for incubation with goat-derived primary antibodies, or 3% BSA blocking buffer for incubation with primary antibodies from other species) were added, and incubation was carried out at room temperature for 30 min. Pre-prepared primary antibody anti-v5 (1:200, CST, USA) was added, and the slides were incubated flat in a humidified chamber at 4 °C overnight. ..



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